The publication "Oestrogen-binding protein in Candida albicans: antibody development and cellular localization by electron immunocytochemistry" by X. Zhao, P.J. Malloy, C.M. Ardies, and D. Feldman is a scientific study that investigates the presence of an estrogen-binding protein in Candida albicans, a common fungal pathogen in humans. The research team aimed to develop antibodies for this protein and to determine its cellular localization using electron immunocytochemistry.
Electron immunocytochemistry is a technique that uses antibodies labeled with electron-dense markers to visualize target proteins within cells using an electron microscope. This method allows researchers to identify the precise location of specific proteins at a subcellular level.
In this study, the authors focused on understanding the interaction between Candida albicans and estrogen, a hormone that has been suggested to play a role in the growth and virulence of the fungus. By developing antibodies against the estrogen-binding protein and using electron immunocytochemistry, they aimed to provide insights into the cellular localization of this protein and potentially uncover its role in the pathogenesis of Candida infections.